Abcam, ab267618, Human DPP2 ELISA Kit (DPP7)

Size: 1 x 96Tests
Human DPP2 ELISA Kit (DPP7) is a Sandwich (quantitative) ELISA for the measurement of Human DPP2 (DPP7) in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.11 ng/mL,
Range:0.102 - 25 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human DPP2 ELISA Kit (DPP7) is designed for the quantitative determination of DPP2 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human DPP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and DPP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human DPP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DPP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Dipeptidyl peptidase 2 (DPP2) also known as DPP7 is a serine protease enzyme with a molecular mass of approximately 55 kDa. This enzyme hydrolyzes dipeptides from the N-terminus of polypeptides with a preference for sequences with proline or alanine in the penultimate position. DPP2 is located in the cytoplasmic subcellular regions of cells. It is expressed in various tissues including the spleen thymus and other organs involved in hematopoiesis and immune response.
Biological function summary
DPP2 has an important role in cellular processes related to quiescence and apoptosis managing protein turnover and regulation of the cell cycle. It forms part of larger protein complexes that modulate the intracellular protein degradation system and peptide hormone processing. Furthermore DPP2 contributes to immune function through its regulation of lymphocyte quiescence maintaining cells in a resting state until activation is required.
Pathways
DPP2 takes an active part in the metabolism of regulatory peptides and is involved in immune response pathways. It interacts with proteins such as DPP4 which shares some functional similarities participating in immunomodulatory processes. DPP2's actions are integrated into the broader networks of proteolysis and peptide signal modulation influencing cell communication dynamics and immune surveillance activities.
DPP2 has connections to immune-related conditions and certain types of cancer. Links to leukemia have emerged partly through its role as an apoptosis regulator and potential influence on hematopoietic differentiation. The enzyme is also observed in metabolic disorders due to its similarity with DPP4 a known target for addressing conditions like Type 2 diabetes. Its modulation of immune cell function and apoptosis highlights its significance as a therapeutic target in disease settings where these processes are disrupted.