Abcam, ab267644, Mouse IL-17RB ELISA Kit

Size: 1 x 96Tests
Mouse IL-17RB ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse IL-17RB in Mouse in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.2 ng/mL,
Range:0.2 - 50 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse IL-17RB ELISA Kit is designed for the quantitative determination of IL-17RB in cell culture supernatants, plasma and serum samples.
Δ Note:
Mouse IL-17RB concentration is low in normal serum/plasma and may not be detectable in this assay.
This assay employs an antibody specific for mouse IL-17RB coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17RB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-17RB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17RB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse Adiponectin (ACRP30), ANGPTL1, ANGPTL3, Artemin, CCL28 (MEC), CD36 (SRB3), Chordin, CRP, E-Cadherin, Epigen, Epiregulin, Fas, Galectin-7, gp130, Granzyme B, Gremlin-1, IFN-gamma R1, IL-17B, IL-22, MIP-1beta, MMP-10, MMP-2, MMP-3, PDGF-AA, Persephin, sFRP-3, SLAM, Shh-N, TCK-1, TECK, TGF beta 1, Trance, TREML1, TWEAK, VEGF-B, VEGFR2.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C