Abcam, ab267651, Human Jagged1 ELISA Kit

Size: 1 x 96Tests
Human Jagged1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Jagged1 in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 1.6 ng/mL,
Range:1.638 - 400 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Jagged1 ELISA Kit is designed for the quantitative determination of Jagged1 in cell culture supernatants, plasma and serum samples.
∆Note: H
uman Jagged1 concentration is low in normal serum/plasma, and may not be detectable in this assay.
This assay employs an antibody specific for human Jagged1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Jagged1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Jagged1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Jagged1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit detects human Jagged1. Other species not determined.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Jagged1 also known as JAG1 or Jagged 1 protein is a transmembrane protein involved in the Notch signaling pathway. It has a mass of approximately 150 kDa and is expressed in various tissues including the cardiovascular system liver and mammary glands. Not only does it play a role in cell-cell communication but it also engages in controlling cell differentiation proliferation and apoptosis. Additionally researchers studying its molecular nature frequently employ Jagged1 ELISA kits to measure its expression and activity levels.
Biological function summary
The Jagged1 protein acts as a ligand binding to Notch receptors forming a large complex with other critical proteins within the pathway. This interaction initiates the signal transduction necessary for various cellular processes reinforcing the influence Jagged1 has on development and tissue homeostasis. Its role as a ligand amplifies its importance and scientists often use Jagged1 ELISA and Jag1 ELISA assays to explore these biological interactions further.
Pathways
Research shows that Jagged1 is an integral component of the Notch signaling and cardiovascular development pathways. It binds to Notch1 and Notch2 receptors facilitating important steps in both pathways which are related to embryonic development and adult tissue maintenance. This Notch-Jagged1 interaction also connects to Delta-like ligands creating an intricate network of proteins and pathways critical for a wide spectrum of cellular functions.
Jagged1 exhibits significant connections to Alagille syndrome and some cancers. Mutations in Jagged1 or disrupted interactions with Notch receptors often lead to the developmental defects characteristic of Alagille syndrome where both Jagged1 and Notch2 play significant roles. Additionally aberrant activation of the Notch signaling pathway involving Jagged1 has links to tumorigenesis in certain cancers opining avenues for potential therapeutic interventions against these diseases.