Abcam, ab267659, Human IL-1R3 ELISA Kit

Size: 1 x 96Tests
Human IL-1R3 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IL-1R3 in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture media, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 18 pg/mL,
Range:16.38 - 4000 pg/mL,
Assay Platform:Microplate

Product details:
Human IL-1R3 ELISA Kit is designed for the quantitative determination of IL-1R3 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human IL-1R3 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1R3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-1R3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1R3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C