Abcam, ab267802, Human PAP ELISA Kit (ACPP)

Size: 1 x 96Tests
Human PAP ELISA Kit (ACPP) is a single-wash 90-min Simplestep used to quantify Human PAP (ACPP) with a sensitivity of 9.73 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Urine, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 9.73 pg/mL,
Range:37.5 - 2400 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human PAP ELISA Kit (ACPP) (ab267802) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PAP (ACPP) protein in cell culture supernatant, edta plasma, hep plasma, serum, urine, cit plasma, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PAP (ACPP) with 9.73 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
PAP (Prostatic Acid Phosphatase, EC:3.1.3.2), also known as ACPP is a non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. PAP expression is highest in prostate. PAP is biomarker of prostate cancer as its expression is increased in prostate cancer patients. The isoform 1 is secreted, the isoform 2 is a type I integral membrane protein of plasma membrane and lysosomes. The Isoform 2 function as tumor suppressor through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling. The antibodies of this kit are designed to detect both isoforms.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C