Abcam, ab268696, Recombinant human KAT5 / Tip60 protein (Active)

Size: 5µg
Recombinant human KAT5 / Tip60 protein (Active) is a Human Full Length protein, in the 1 to 513 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:GST tag N-Terminus,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The specific activity of ab268696 was 34 nmol/min/mg in an acetyltransferase assay using histone H4 peptide (1-21) as substrate.,
Accession:Q92993,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.31% Glutathione, 0.29% Sodium chloride, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
KAT5 also known as Tip60 is a histone acetyltransferase with a molecular mass of approximately 60 kDa. This enzyme plays an important mechanical role in regulating chromatin dynamics by acetylating lysine residues on histone tails which affects gene expression. It is also involved in non-histone protein acetylation influencing various signaling pathways. KAT5 is expressed across many tissues with higher levels observed in the brain heart and testis.
Biological function summary
KAT5 acetylates histones and participates in the regulation of transcription DNA repair and cell cycle progression. It is a critical component of the NuA4 complex which includes other important proteins like EP400 and TRRAP. Through its acetylation activity KAT5 affects chromatin structure thereby influencing gene expression involved in growth control apoptosis and DNA repair mechanisms.
Pathways
KAT5 is integrally involved in processes such as the DNA damage response and apoptosis. Within these pathways KAT5 interacts with proteins like p53 which plays a significant role in maintaining genomic stability. KAT5-mediated acetylation modulates p53's activation and its ability to engage in apoptotic pathways as well as the cell's response to DNA damage.
KAT5 has links to neurodegenerative disorders and cancer. Its dysregulation may contribute to the development of Alzheimer's disease through altered acetylation of tau protein; abnormalities in tau acetylation are implicated in this disorder. Similarly in cancer interactions with proteins like MYC suggest KAT5's involvement in oncogenic pathways. Changes in KAT5 expression or activity have been observed in certain cancers highlighting its potential as a therapeutic target.