Abcam, ab268857, Recombinant human PI3 Kinase p110 beta + PI 3 Kinase p85 beta protein (Active)

Size: 5µg
Recombinant human PI3 Kinase p110 beta + PI 3 Kinase p85 beta protein (Active) is a Human Full Length protein, in the 1 to 728 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:Tag free,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The specific activity of ab268857 was 360 nmol/min/mg in a kinase assay using a PI(4,5)P2:PS mixture as substrate.,
Accession:P42338,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7Preservative: 1.02% ImidazoleConstituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

Product details:
Subunits co-expressed.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PI3 Kinase p110 beta also known as p110β and PI 3 Kinase p85 beta form part of a heterodimer complex where the p110β serves as the catalytic unit and p85β as the regulatory subunit. The molecular mass of p110β is approximately 122 kDa. These proteins are components of the phosphoinositide 3-kinase (PI3K) pathways and their expression occurs broadly across different tissues in the human body. Both proteins have a strong presence in tissues such as the liver and fat wherein they modulate various cellular functions.
Biological function summary
These subunits interact closely to regulate important cellular processes including cell growth metabolism and survival signals. The heterodimer acts as a vital signaling entity that helps mediate the response to extracellular signals. Through their interaction with regulatory and catalytic subunits they modulate the lipid kinase activities contributing to cellular proliferation and glucose uptake. The complex nature of their interactions ensures precise regulation of downstream signaling pathways.
Pathways
The PI3 Kinase pathway is central to many intracellular signaling routes notably the AKT signaling pathway. This association influences processes like cell cycle progression and apoptosis control. Apart from AKT they also connect with related proteins such as PTEN an important negative regulator that helps maintain balance within the pathway. The engagement of p110β and p85β in these pathways demonstrates their roles in translating external signals into diverse cellular responses.
Altered activity of PI3 Kinase p110 beta and PI 3 Kinase p85 beta can lead to various conditions including cancer and diabetes. These proteins often exhibit dysregulated signaling in tumorigenesis where their interactions with oncogenic proteins like PTEN become disrupted. Similarly in diabetes impaired signaling pathways involving these subunits can affect glucose metabolism. The sensitive regulation of these proteins holds significant importance in understanding their roles in disease mechanisms.