Abcam, ab268862, Recombinant human PI4KA protein (Active)

Size: 5µg
Recombinant human PI4KA protein (Active) is a Human Full Length protein, in the 1 to 2102 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>70% Densitometry,
Expression system:Baculovirus infected Sf9 cells,
Tags:GST tag N-Terminus,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The specific activity of ab268862 was 70 nmol/min/mg in a kinase assay using PI:PS as substrate.,
Accession:P42356,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PI4KA also known as phosphatidylinositol 4-kinase alpha is an enzyme responsible for phosphorylating phosphatidylinositol to produce phosphatidylinositol 4-phosphate (PI4P). The protein has a mass of approximately 230 kDa. PI4KA is expressed broadly with significant levels in the brain heart and other tissues. It plays an important role as a regulator of cellular lipid metabolism and intracellular signaling.
Biological function summary
PI4KA functions in the context of membrane dynamics and acts as part of a multi-subunit complex. This protein is important in maintaining the proper lipid composition of the Golgi apparatus and other organelles which is essential for vesicle trafficking. By generating PI4P PI4KA serves as an important mediator in the distribution and dynamics of cellular membranes aiding in various cellular processes including endocytosis and autophagy. Its activity impacts several downstream signaling pathways and cellular activities.
Pathways
PI4KA is a significant contributor to the phosphoinositide signaling pathway which plays an essential role in regulating intracellular processes. It interacts with phosphatidylinositol-45-bisphosphate (PIP2) and phospholipase C supporting the synthesis of further derivatives that modulate calcium influx and other cellular responses. Additionally PI4KA is involved in the maintenance and function of the secretory pathway where it coordinates with proteins such as Osh1 and CERT influencing lipid exchange and protein trafficking.
PI4KA's dysfunction has been linked to neurological disorders and cancer. Alterations in its activity can contribute to conditions like neurodegenerative diseases due to disrupted membrane composition in neural tissues. Furthermore PI4KA's role in cancer is illuminated through its interaction with proteins like AKT where aberrant PI4P levels disrupt normal cell growth signals leading to oncogenesis. Understanding PI4KA's pathways and interactions is critical as they offer potential therapeutic targets for these diseases.