Abcam, ab268865, Recombinant human PIK3C2B protein (Active)

Size: 5µg
Recombinant human PIK3C2B protein (Active) is a Human Fragment protein, in the 850 to 1606 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>70% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:GST tag N-Terminus,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The specific activity of ab268865 was 100 nmol/min/mg in a kinase assay using PI:PS as substrate.,
Accession:O00750,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.31% Glutathione, 0.29% Sodium chloride, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PIK3C2B also known as phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing beta polypeptide is a type II phosphoinositide 3-kinase with a molecular mass of approximately 187 kDa. This enzyme is part of the PI3K family which plays essential roles in cell signaling pathways. PIK3C2B is mostly present in human tissues such as the liver heart and brain where it functions in various physiological processes. In terms of structure it possesses both a C2 domain aiding in cell membrane interaction and a helical domain that assists in catalysis.
Biological function summary
The enzyme phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing beta polypeptide plays a significant role in the regulation of endosomal trafficking and cell migration by phosphorylating phosphoinositides. PIK3C2B can also impact insulin signaling and GLUT4 translocation in cells contributing to glucose metabolism. It forms part of a larger protein complex that aids its action in cellular environments. Its interactions with small GTPases suggest that it is involved in cellular morphology and dynamics.
Pathways
Phosphoinositide-4-phosphate 3-kinase functions significantly in the PI3K/Akt signaling pathway affecting cellular growth and survival. It also plays a role in the insulin signaling pathway where it works alongside proteins like PIK3CA and PTEN in regulating cellular responses to hormones. Through the PI3K/Akt pathway PIK3C2B modulates vital processes such as the cell cycle and apoptosis highlighting its contribution to maintaining cellular homeostasis and response to external signals.
The enzyme phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing beta polypeptide associates with metabolic disorders and cancers. Alterations in its expression or function can lead to aberrant signaling in insulin resistance conditions like type 2 diabetes. Additionally due to its role in cell proliferation pathways PIK3C2B induces oncogenic processes when dysregulated. Connections with proteins such as Akt and mTOR further emphasize its relevance in these diseases offering potential therapeutic targets for intervention.