Abcam, ab268873, Recombinant Human PIP5K3/PIKFYVE protein (Tagged)

Size: 20µg
Recombinant Human PIP5K3/PIKFYVE protein (Tagged) is a Human Fragment protein, in the 1493 to 2098 aa range, expressed in Baculovirus infected Sf9 cells, with >75%, suitable for SDS-PAGE, WB.
Key facts
Purity:>75% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:GST tag N-Terminus,
Applications:SDS-PAGE, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q9Y2I7,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.31% Glutathione, 0.29% Sodium chloride, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PIP5K3 also known as PIKFYVE is a lipid kinase with a significant role in the phosphorylation of phosphatidylinositol 3-phosphate to phosphatidylinositol (35)-bisphosphate. This protein weighs approximately 237 kDa and is predominantly expressed in the cytoplasm of a variety of cell types. Its enzymatic activity is essential for the maintenance of phosphatidylinositol 35-bisphosphate levels influencing membrane trafficking and endosomal function.
Biological function summary
PIP5K3/PIKFYVE functions as an important component in regulating endosomal dynamics. It is part of a complex with VAC14 and FIG4 which plays a significant role in endosomal membrane trafficking lysosome biogenesis and autophagy. The protein's expression aids in maintaining cellular homeostasis and modulating organelle identity within the endosomal-lysosomal system which is critical for normal cellular operations.
Pathways
PIP5K3/PIKFYVE integrates into the phosphatidylinositol signaling pathway and is important for proper endocytic trafficking. This protein interacts with key pathway constituents like Rab5 and Rab7 facilitating the transport of endosomes and impacting the process of autophagy. These interactions highlight the protein's centrality in the coordination of intracellular trafficking processes essential for cellular health.
PIP5K3/PIKFYVE mutations and dysregulation associate with rare disorders such as Charcot-Marie-Tooth disease and fleck corneal dystrophy. Abnormal functioning of PIP5K3/PIKFYVE can affect its complex partners FIG4 and VAC14 leading to neurodegenerative symptoms and defects in cell morphology. Studies reveal vulnerabilities in cellular pathways due to aberrations in this protein offering potential targets for therapeutic intervention.