Abcam, ab268985, Recombinant human KMT1A / SUV39H1 protein

Size: 5µg
Recombinant human KMT1A / SUV39H1 protein is a Human Full Length protein, in the 1 to 412 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>70% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:GST tag N-Terminus,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The specific activity of ab268985 was 2.2 nmol/min/mg in methyltransferase assay using a Histone H3 peptide substrate.,
Accession:O43463,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
KMT1A also known as SUV39H1 is a well-studied histone methyltransferase responsible for trimethylating histone H3 at lysine 9 (H3K9me3) an important marker for heterochromatin formation. This enzyme plays a critical role in chromatin organization and gene regulation. KMT1A has a molecular weight of approximately 48 kDa. Researchers have observed its expression across a range of tissues including the brain heart and muscle which suggests its importance in various physiological processes.
Biological function summary
KMT1A functions as part of a Suv39H1/HP1 complex that maintains the integrity of pericentric heterochromatin essential for chromosome stability during cell division. Its influence extends over the transcriptional silencing of specific genes by modifying chromatin structure to a closed state. KMT1A also collaborates with other proteins like HP1 to silence repetitive elements and prevent genomic instability which is often important for safeguarding genomic fidelity in cells.
Pathways
Researchers identify KMT1A as a significant player in the regulation of epigenetic pathways particularly the histone methylation process. It interacts closely with other histone methyltransferases and demethylases contributing to a dynamic epigenetic landscape that controls gene expression. Additionally KMT1A is involved in the DNA damage response pathway in collaboration with proteins like ATM which coordinates repair mechanisms to maintain genetic stability and respond to cellular stress.
Researchers have linked KMT1A with cancer and neurodegenerative diseases such as Huntington’s disease. Abnormal expression or function of KMT1A can lead to disrupted heterochromatin structure and uncontrolled gene expression which contributes to tumorigenesis. Furthermore in the context of neurodegenerative conditions interactions with proteins such as HTT can exacerbate neuronal damage and cell death. Studying these associations provides insights into potential therapeutic targets for managing such diseases.