Abcam, ab269036, Recombinant GST-TRK fused gene-ALK fusion protein (Active)

Size: 5µg
Recombinant GST-TRK fused gene-ALK fusion protein (Active) is a Human Fragment protein, in the 1 to 138 aa range, expressed in Baculovirus infected Sf9 cells, with >85%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>85% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:Tag free,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The specific activity of ab269036 was 22 nmol/min/mg in a kinase assay using IGF1Rtide synthetic peptide substrate.,
Accession:Q92734,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Product details:
GST-TRK fused gene-ALK fusion protein.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The ALKTRK fused gene product known as a fusion protein combines parts of the anaplastic lymphoma kinase (ALK) and neurotrophic receptor tyrosine kinase (TRK) genes. The fusion protein has a molecular mass usually in the range of 75–200 kDa dependent on the exact fusion partners and the number of amino acids in the resulting protein. Researchers identify these proteins mainly in cancer cells where they play a role in oncogenic signaling. ALK typically shows expression in the brain and nervous system while TRK proteins express in the nervous system and various other tissues during development.
Biological function summary
The ALKTRK fusion protein participates in abnormal cellular signaling that can lead to uncontrolled cell growth. Normally both ALK and TRK proteins function in neuronal development and signaling but when fused the resulting protein loses normal regulatory control. This fusion protein is not generally part of a larger complex but acts independently to drive oncogenic processes. ALK a receptor tyrosine kinase activates pathways that promote cell proliferation and survival mimicked in the fusion form with TRK proteins.
Pathways
The ALKTRK fusion protein engages primarily in the MAPK/ERK and PI3K/AKT pathways which are critical for cell proliferation and survival. In its normal non-fused state ALK interacts in pathways with proteins like IRS1 and GRB2 while TRK partners with proteins such as SHC and PLC-γ. The fusion form activates similar downstream signaling pathways bypassing normal cellular controls and frequently leading to malignancy.
The ALKTRK fused gene product associates closely with certain cancers particularly non-small cell lung cancer and some pediatric malignancies. This fusion protein plays a significant role in these diseases by driving oncogenic transformation and progression. Specifically TRK fusions have been implicated in various solid tumors while ALK fusions are prominently observed in anaplastic large cell lymphoma. Targeting the ALKTRK fusion protein with specific inhibitors can lead to effective therapies for these cancers.