Abcam, ab269074, Recombinant human TrkA (mutated G595R) protein (Active)

Size: 5µg
Recombinant human TrkA (mutated G595R) protein (Active) is a Human Fragment protein, in the 440 to 796 aa range, expressed in Baculovirus infected Sf9 cells, with >85%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>85% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:GST tag N-Terminus,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The specific activity of ab269074 was 2.7 nmol/min/mg in a kinase assay using Poly (4:1 Glu, Tyr) synthetic peptide as substrate.,
Accession:P04629,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TrkA also known as NTRK1 is a neurotrophin receptor and a member of the tropomyosin receptor kinase (TRK) family. It has a molecular mass of approximately 140 kDa. TrkA is expressed mostly in neuronal tissues particularly in the sensory ganglia sympathetic neurons and certain portions of the central nervous system. Mechanically TrkA activates upon binding to nerve growth factor (NGF) leading to receptor dimerization and autophosphorylation triggering downstream signaling cascades essential for neuronal growth and survival.
Biological function summary
TrkA plays an important role in the regulation of neurotrophic signaling. TrkA existing often as part of larger signaling complexes modulates neuronal differentiation development and apoptosis. It ensures proper neural connections during development by responding to NGF which is important for neurons' health and plasticity. TrkA also aids in the response to injury by promoting neuronal repair and regeneration.
Pathways
TrkA engagement activates prominent pathways like the MAPK/ERK and PI3K/AKT signaling cascades. These pathways facilitate important cellular processes like proliferation survival and metabolism. Through these pathways TrkA interacts and cooperates with proteins like SHC GRB2 and PLCγ among others to execute cellular functions. These signaling pathways enable the coordinated response needed for neural tissue maintenance and development.
TrkA abnormalities are linked to conditions like congenital insensitivity to pain with anhidrosis (CIPA) and certain cancers. Mutations in NTRK1 can disrupt NGF signaling leading to neurological deficits observed in CIPA. In cancer aberrant TrkA signaling often due to gene fusions contributes to tumorigenesis. TrkA-related signals influence oncogenic proteins such as RAS and BRAF illustrating its role in cancer progression.