Abcam, ab269128, Recombinant human UBPY/USP8 protein (Active)

Size: 5µg
Recombinant human UBPY/USP8 protein (Active) is a Human Fragment protein, expressed in Escherichia coli, with >70%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>70% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:ab269128 specific activity was determined to be 80 nmol/min/mg in a DUB assay using recombinant human ubiquitin-based proluciferin substrate.,
Accession:P40818,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7Preservative: 1.02% ImidazoleConstituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
UBPY also known as USP8 (ubiquitin-specific-processing protease 8) functions as a deubiquitinating enzyme. This protein removes ubiquitin from specific substrates regulating their stability and turnover. USP8 has a molecular mass of approximately 125 kDa. It is widely expressed in various tissues with higher expression levels reported in the brain liver and kidneys. The enzyme plays a significant role in protein homeostasis and cellular signaling through its deubiquitinating activity.
Biological function summary
The deubiquitinating enzyme USP8 participates in endocytosis and lysosomal protein degradation processes. It does not function alone but associates with different protein complexes to regulate trafficking and degradation of membrane receptors. Through its action USP8 influences receptor tyrosine kinases like EGFR and impacts the sorting and degradation of these receptors. The enzyme's activity on these receptors is critical for controlling their signal transduction pathways and cellular responses.
Pathways
USP8 plays a role in the EGFR signaling pathway and the Wnt pathway. In the EGFR pathway its deubiquitinating action on EGFR affects receptor degradation and recycling impacting cell growth and differentiation. Within the Wnt pathway USP8 associates with proteins such as Axin and APC contributing to the regulation of β-catenin levels. By managing these pathways USP8 controls both normal physiological processes and pathological conditions when dysregulated.
USP8 associates with conditions like Cushing's disease and certain types of cancers. Cushing's disease results from pituitary adenomas overexpressing USP8 leading to excessive adrenocorticotropic hormone (ACTH) production. In cancer the regulation of EGFR by USP8 becomes disrupted resulting in unregulated cell growth and proliferation. These disorders highlight the important role of USP8 in maintaining cellular homeostasis and the potential of targeting this enzyme for therapeutic interventions.