Abcam, ab269579, Anti-BirA antibody [6C4c7] - BSA and Azide free

Size: 100µg
Mouse Monoclonal BIRA antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Escherichia coli samples.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:6C4c7,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:Yes,
Reacts with:Escherichia coli,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab269579 is the carrier-free version of
ab232732
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein BirA also known as the biotin protein ligase (BPL) serves an important role in biotinylation. Mechanically BirA activates biotin through ATP-dependent reactions linking it to proteins. The BirA protein has an approximate molecular weight of 35 kDa a characteristic referred to as the BirA molecular weight or BirA protein size. BirA is typically expressed in bacterial cells with Escherichia coli being a primary model organism for studying its activity. In its active form BirA functions as a homodimer a stable complex required for its catalytic activity.
Biological function summary
BirA adds biotin to specific lysine residues on target proteins a modification important for protein function. This process often regulates proteins involved in metabolism and gene expression. BirA does not operate as a part of a larger protein complex but acts on its own enzyme-substrate interactions. Its distribution is notable in prokaryotic cells where it plays essential roles in microbial biotin metabolism.
Pathways
BirA significantly interacts in the biotin metabolic pathway vital for fatty acid synthesis and gluconeogenesis. The protein's ability to biotinylate targets is vital in pathways where it helps regulate enzymes like acetyl-CoA carboxylase and pyruvate carboxylase which are critical for lipid and carbohydrate metabolism. In the biotin regulation network BirA interacts with other proteins including the biotin repressor protein BioR for controlling gene expression related to biotin and related pathways.
Deficiencies in biotin metabolism pathways where BirA activity is central can lead to conditions such as biotinidase deficiency and phenylketonuria (PKU). Disruptions in these pathways often result from inadequate biotinylation where BirA plays a critical role. Although not directly linked to human diseases the homologs of BirA studied in human cells are relevant for understanding the molecular basis of metabolic disorders.