Abcam, ab270561, Anti-Cytochrome C antibody [6H2.B4] - BSA and Azide free

Size: 100µg
Mouse Monoclonal Cytochrome C antibody. Carrier free. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Immunogen corresponding to Full Length Protein corresponding to Rat Cycs.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:6H2.B4,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:Yes,
Reacts with:Human,
Applications:ICC/IF, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Full Length Protein corresponding to Rat Cycs. The exact immunogen used to generate this antibody is proprietary information.P62898

Product details:
ab270561 is a carrier free version of
ab270249
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A/G, Purification notes-Purified from bioreactor concentrate., Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cytochrome C also referred to as "cytochrome c" or "Cyt C" is a small protein with a molecular weight of approximately 12 kDa. It is a component of the electron transport chain playing an essential mechanical role in cellular respiration. Cytochrome C is expressed in the mitochondria of eukaryotic cells where it functions as a heme protein. The mass of this protein allows efficient electron transfer between Complex III (cytochrome bc1 complex) and Complex IV (cytochrome c oxidase) contributing to ATP synthesis.
Biological function summary
Cytochrome c contributes to the process of oxidative phosphorylation within mitochondria. It is not only an important electron carrier but it also plays a significant role in apoptosis. In the event of cellular stress cytochrome c releases into the cytosol and forms part of the apoptosome complex. This apoptosome activation leads to the caspase cascade driving programmed cell death. Therefore cytochrome C serves as both a mediator of energy production and a regulator of apoptosis.
Pathways
Cytochrome c is integral to the mitochondrial electron transport chain and apoptotic pathways. It interacts with proteins in these pathways such as cytochrome c oxidase in oxidative phosphorylation and Apaf-1 during apoptosis. The electron transport chain's function is dependent on the efficient transfer of electrons facilitated by cytochrome c ensuring ATP production in cellular respiration.
Cytochrome c links to conditions like cancer and neurodegenerative diseases. In cancer its role in the apoptotic pathway often emerges as cancer cells can develop mechanisms to evade apoptosis impacting cytochrome c release. For neurodegenerative diseases disruptions in mitochondrial function and cytochrome c's release can facilitate cell death implicating its role in conditions like Parkinson's disease. The interaction of cytochrome c with proteins such as p53 in response to cellular stress further highlights its involvement in disease pathogenesis.