Abcam, ab270883, Human IL-2 ELISA Kit

Size: 1 x 96Tests / 1 x 384Tests / 10 x 96Tests
Human IL-2 ELISA Kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify human interleukin-2 (IL-2) with a sensitivity of 32.1 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step. - Colorimetric Sandwich ELISA - 450 nm readout; compatible with any standard plate reader - Available in different formats to meet various throughput needs, including 384-well plates for higher throughput - Broad sample compatibility – suitable for cell culture media, cell culture supernatant, serum and plasma - Cited in over 10 publications
Key facts
Detection method:Colorimetric,
Sample types:Cell culture media, Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 32.1 pg/mL,
Range:39 - 2500 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human IL-2 ELISA Kit ab270883 is a rapid single-wash 90-min ELISA kit to measure Human IL-2 in cell culture media, cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 32.1 pg/mL.
How the assay works
Human IL-2 SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and recombinant human IL-2 protein in stimulated serum, plasmas, and cell culture supernatant. Urine, milk, saliva, and cell and tissue extract samples have not been tested with this kit. Recombinant human IL-2R alpha was prepared at 5,000 pg/mL and assayed for cross reactivity. No cross-reactivity was observed. Recombinant human IL-2R alpha was prepared at 5,000 pg/mL and tested for interference. No interference with was observed.
Human IL-2 ELISA Kit ab270883 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human IL-2 ELISA Kit ab270883
Human IL-2 ELISA Kit ab270883 has been used to study the role of long noncoding RNA in immunotherapy resistance
and exosomal effects of HLA transfer on T lymphocytes in Celiac Disease
References:
Hui-Yan Luo et al. 2023, PMID: 37712124,
Valerie Brid O'Leary et al. 2022, PMID: 35682780
Save your precious samples
Our 384-well Human IL-2 ELISA Kit only requires a maxmum of 12.5µL
Related and recommended products
Human IL-2 ELISA Kit ab270883 is commonly used to measure human interleukin 2 as a marker of inflammation.
Other ELISA kits to measure inflammatory markers include:
- Human TNF alpha ELISA kit
ab181421
- Human IL-6 ELISA kit
ab178013
- Human IFN gamma ELISA kit
ab174443

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C