Abcam, ab270892, Mouse GPNMB ELISA Kit

Size: 1 x 96Tests
Mouse GPNMB ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse GPNMB with a sensitivity of 362 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell culture media, Citrate plasma, Serum, EDTA Plasma,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 362 pg/mL,
Range:0.78 - 50 ng/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse GPNMB ELISA kit (ab270892) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GPNMB protein in mouse serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate mouse GPNMB with 362 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
GPNMB (Glycoprotein non-metastatic gene B), also known as Osteoactivin, is a type I transmembrane glycoprotein most often associated with endosomal/lysosomal compartment and over expressed in many types of cancer. GPNMB is involved in many physiological processes, including transport of late melanosomes to keratinocytes, regulation of osteoblast and osteoclast, stimulation of dendritic cell maturation, and stimulation of adhesion of dendritic cells to endothelial cells.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GPNMB also known as osteoactivin is a type I transmembrane glycoprotein with a molecular mass of approximately 115 kDa. This protein is highly expressed in various tissues including the bone skin and brain. It also shows notable presence in certain immune cells such as dendritic cells and macrophages. GPNMB localizes mainly on the cell membrane and within lysosomal compartments indicating its involvement in cellular and signaling processes.
Biological function summary
GPNMB plays versatile roles in promoting cell growth differentiation and repair. It is involved in processes like tissue regeneration possibly due to its role in cell adhesion and migration. The protein also partakes in osteoclast differentiation and bone resorption therefore linking to skeletal homeostasis. GPNMB forms part of a broader protein complex at the cellular junctions interacting with integrins to mediate its functions.
Pathways
GPNMB features prominently in osteoclastogenesis and melanoma metastasis pathways. It cooperates with RANKL an important protein in bone metabolism by influencing osteoclast differentiation and activation. In the context of melanoma GPNMB's action in pathways may enhance tumor proliferation and invasion possibly interacting with signaling molecules like AKT or ERK although the specifics remain actively researched.
GPNMB has significant links to both osteoarthritis and certain types of cancer particularly breast cancer and melanoma. In osteoarthritis GPNMB expression often increases reflecting involvement in cartilage degradation processes. Its overexpression correlates with tumor progression in melanoma potentially driven by its interaction with integrin β1 which assists in cellular adhesion and migration within the tumor microenvironment.