Abcam, ab271002, Anti-DDX3 antibody [EPR24221-72]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal DDX3 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples. Cited in 3 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24221-72,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Rat, Mouse,
Applications:ICC/IF, Flow Cyt (Intra), IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DDX3 also known as DEAD-box helicase 3 plays an important role mechanically as an RNA helicase involved in unwinding RNA secondary structures. The protein has a mass of approximately 73 kDa and is commonly expressed in the cytoplasm and nucleus of a wide range of cell types. Other names for DDX3 include DBX CAP-Rf and DDX3X highlighting its varied functional roles across different studies.
Biological function summary
DEAD-box helicase 3 serves as a multifunctional protein that regulates gene expression and is implicated in RNA splicing transport and translation. It forms part of various ribonucleoprotein complexes influencing processes such as mRNA export and translation initiation. DDX3 often interacts with other proteins like eIF4E which further modulates translation of specific mRNAs.
Pathways
DEAD-box helicase 3 acts as an essential component in several cellular pathways. It plays roles in the Wnt/β-catenin signaling pathway where it can influence gene transcription important for cell fate decisions. DDX3 also participates in the innate immune response pathway interacting with proteins like MAVS to modulate antiviral defense mechanisms.
DEAD-box helicase 3 has been associated with various cancers including hepatocellular carcinoma and breast cancer. Abnormal DDX3 function or expression can disrupt cell cycle regulation leading to uncontrolled cell growth. Additionally in viral infections such as Hepatitis C DDX3 interacts with viral proteins to facilitate replication showcasing its role in both promoting and hindering disease progression.