Abcam, ab271286, Anti-CD63 antibody [KILL150A]

Size: 100µg / 1mg
Anti-CD63 antibody [KILL150A] (ab271286) is a mouse monoclonal antibody detecting CD63 in Western Blot, Flow Cytometry, IHC-P, ICC/IF . Suitable for Human . - Over 20 publications
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:KILL150A,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt, WB, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:We recommend ab134045 for low expression samples.

Product details:
What is this antibody validated in?
Anti-CD63 antibody [KILL150A] (ab271286) is a mouse recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of CD63?
Anti-CD63 [KILL150A] (ab271286) specifically detects a band for CD63 (UniProt: P08962) at a molecular weight of 26kDa.
Trusted by the scientific community
Anti-CD63 [KILL150A] (ab271286) was first used in a scientific publication in 2020 and has been cited over 20 times in peer-reviewed journals.
Other related products
We have a range of other formats of antibody clone [KILL150A] also available for your convenience: ab271286, Carrier free -
ab271296
, Alexa Fluor® 488 -
ab318246
, PE -
ab322728
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD63 is a protein commonly known as LAMP-3 a member of the tetraspanin family with a molecular weight of approximately 25-65 kDa depending on post-translational modifications such as glycosylation. It resides mainly on the membrane of intracellular vesicles such as lysosomes and platelets. CD63 also appears on the surface of activated cells particularly in immune cells. Researchers often use CD63 as a marker in assays including CD63 western blot techniques due to its distinct expression patterns.
Biological function summary
CD63 participates in various cellular processes including membrane trafficking cell adhesion and signal transduction. It associates with integrins and other tetraspanins forming complexes that influence cellular adhesion and migration. Through its role in exosome formation CD63 contributes significantly to intercellular communication by facilitating the transfer of proteins lipids and RNA between cells.
Pathways
CD63 plays a role in pathways linked to cell proliferation and migration and immune response regulation. Within the integrin-mediated signaling pathway CD63 interacts with proteins such as integrins to promote cell adhesion and migration. CD63 also links to vesicle trafficking pathways collaborating with proteins involved in exocytosis and endocytosis which affect cell membrane restructuring and intracellular transport.
CD63 shows connections with cancer and immune system disorders. Its role in promoting cell adhesion and migration links it with cancer metastasis including melanoma and breast cancer where CD63 expression levels often rise. Additionally CD63 associates with allergic reactions due to its involvement in histamine release where it impacts immune proteins like FcεRI which participate in allergy pathogenesis.