Abcam, ab271467, Recombinant Human CRBN + DDB1 + CUL-4A + RBX1 protein (Tagged)

Size: 10µg
Recombinant Human CRBN + DDB1 + CUL-4A + RBX1 protein (Tagged) is a Human Full Length protein, in the 1 to 1140 aa range, expressed in HEK 293 cells, with >90%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>90% SDS-PAGE,
Expression system:HEK 293 cells,
Tags:Tag free,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Cereblon-BRD3 interaction was assessed using A-screen technology. Various amounts of ab271467 were incubated in 10-ul reaction with dBET1 and GST-BRD3(BD2) at room temperature for one hour. After this, FLAG-acceptor beads and GSH-donor beads were added followed by detection of A-counts.,
Accession:P62877,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

Product details:
Co-expressed in HEK-293 cells.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The DDB1-CUL4A-RBX1-CRBN complex often referred to simply as CRBN complex plays a core role in targeting proteins for ubiquitination and proteasomal degradation. DDB1 (damage-specific DNA binding protein 1) and CRBN (cereblon) contribute importantly to the functionality of this complex. DDB1 protein weighs about 127 kDa. CRBN is an important component with a mass of about 51 kDa. This complex is highly expressed in various human tissues including the brain liver and lungs. The complex serves as an E3 ubiquitin ligase controlling protein homeostasis.
Biological function summary
The DDB1 CRBN and associated proteins interact closely in the ubiquitin-proteasome system functioning as a part of the larger cullin-RING ligase complex. It acts by recognizing and binding target proteins tagging them for degradation which mediates cellular processes such as DNA repair cell cycle and apoptosis. DDB1 acts as an adaptor that recruits substrate-specific receptors and CRBN often serves as a specificity factor within the protein assembly.
Pathways
The DDB1-CRBN complex intricately integrates into the ubiquitin-proteasome pathway and the cell cycle regulation pathway. It is important for the removal of misfolded or damaged proteins preventing protein aggregation. It interacts with proteins such as c-JUN and MDM2 which link it to cell cycle regulation and stress response pathways. This complex functions in substrate recognition and subsequent degradation to maintain cellular equilibrium.
The dysfunction or deregulation of the DDB1-CUL4A-RBX1-CRBN system associates with cancers and certain neurodegenerative diseases. In hematological malignancies improper CRBN function affects protein homeostasis contributing to disease progression. CRBN is also implicated in certain forms of cognitive dysfunctions where its disrupted function affects neurological signaling pathways. Therapeutic strategies often target this complex indirectly highlighting its potential role in disease treatment alongside proteins like p53 and Cyclin E.