Abcam, ab271469, Recombinant rat CERK protein (Active)

Size: 10µg
Recombinant rat CERK protein (Active) is a Full Length protein, in the 1 to 531 aa range, expressed in Baculovirus infected Sf9 cells, with 65%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:65% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:DDDDK tag C-Terminus,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Specific activity ≥20 pmole/min/μg.CERK activity was measured at 37oC for 25 min in a buffer (20 mM HEPES pH7.2, 80 mM KCl, 3 mM CaCl2, 10 mM MgCl2, 2 mM DTT, 5 μM ATP) using a μ-Octylglucoside mixed micellar assay with 100 μM Ceramide.,
Animal free:No,
Carrier free:No,
Storage buffer:pH: 8Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.02% Potassium chloride

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CERK or ceramide kinase is an enzyme responsible for the conversion of ceramide into ceramide-1-phosphate (C1P). This enzyme has a molecular mass of approximately 66 kDa. Researchers identify CERK as 'beta CERK' due to isoform differences in some cases. CERK expression is observed in several human tissues with significant levels in the brain lungs and spleen. Under physiological conditions CERK localizes mostly to the Golgi apparatus and other intracellular membranes.
Biological function summary
CERK plays a vital role in maintaining the balance of lipid signaling molecules within cells. It acts as an important mediator in the conversion process that produces C1P a molecule involved in signaling pathways that regulate cell survival proliferation and inflammatory responses. While not part of a larger protein complex CERK often interacts with other signaling lipids and proteins to execute its biological functions effectively.
Pathways
CERK is an important component of the ceramide signaling pathway and the sphingolipid metabolism pathway. Within these pathways CERK works alongside sphingosine kinase and other enzymes to regulate the balance of sphingolipids which are critical for cell signaling and membrane structure. The signaling pathway involving CERK influences cellular responses by altering lipid signaling molecules affecting cellular dynamics including apoptosis and growth processes.
CERK has been linked to inflammatory diseases and cancer. Inflammatory disorders like rheumatoid arthritis exhibit altered CERK activity and expression affecting C1P signaling in the inflammatory processes. In cancer aberrant regulation of CERK can modify tumor cell survival and proliferation. This connection highlights its interaction with proteins such as sphingosine-1-phosphate in the disease mechanism indicating potential therapeutic targets for interventions.