Abcam, ab271472, Recombinant human MAP3K8/COT protein (Active)

Size: 10µg
Recombinant human MAP3K8/COT protein (Active) is a Human Fragment protein, in the 30 to 397 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>95% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:GST tag N-Terminus His tag N-Terminus,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Specific activity = 47 pmol/min/μg.100 μl of 240 nM biotinylated MEK1 peptide was coated onto a neutravidin plate for 1 hour at 37°C. After overnight blocking with TBS superblock, the reaction was performed with 10 μM ATP for 60 minutes at RT, and phospho-MEK1 was detected using an anti-phospho-MEK1 antibody. Relative specific activity was determined using a standard kinase under the same conditions.,
Accession:P41279,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.4% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.05% Sorbitan monolaurate, ethoxylated

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information-Avoid freeze / thaw cycle