Abcam, ab271785, Recombinant human KDM6A / UTX protein (Active)

Size: 20µg
Recombinant human KDM6A / UTX protein (Active) is a Human Full Length protein, in the 1 to 1401 aa range, expressed in Baculovirus infected Sf9 cells, with >58%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>58% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:DDDDK tag N-Terminus,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Specific Activity: 0.096 pmol/min/μg.Assay Conditions: 10 μl reaction mix containing assay buffer with 50 mM HEPES (pH 7.4), 1 mM TCEP, 100 μM alpha-ketoglutarate, 25 μM iron, 0.1 mM ascorbic acid, 0.02% Tween-20, 0.5 μM biotinylated peptide substrate, and ab271785 (10 – 50ng) added to the wells. Add antibody against di-methylated K27 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.,
Accession:O15550,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride, 0.01% TCEP HCl

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information-Avoid freeze / thaw cycle