Abcam, ab271808, Recombinant human UBE1 protein (Active)

Size: 100µg
Recombinant human UBE1 protein (Active) is a Human Full Length protein, in the 2 to 1058 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:DDDDK tag N-Terminus,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Reaction was performed in 50 mM Tris pH 7.4, 5 mM MgCl2, 1 mM DTT, 5 μM ATP, 10 μM Ub, and 0.05% Tween. Reaction was initiated with the addition of UBE1 and was incubated at 30°C for 45 mins. The luminescence was read with the Kinase Glo Max reagent.,
Accession:P22314,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information-Avoid freeze / thaw cycle