Abcam, ab271963, Anti-SIK1 (pT182) + SIK2 (pT175) + SIK3 (pT163) antibody [EPR19196] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal SIK2 phospho T175 antibody. Carrier free. Suitable for Dot, WB and reacts with Synthetic peptide - Human, Transfected cell lysate - Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR19196,
Isotype:IgG,
Carrier free:Yes,
Applications:Dot, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Expected to react with Snf1lk (when phosphorylated at T182) and SIK3 ((when phosphorylated at T163).

Product details:
ab271963 is the carrier-free version of
ab199474
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SIK1 SIK2 and SIK3 also known as salt-inducible kinases are serine/threonine protein kinases that play a role in cellular signaling. These proteins have molecular weights of approximately 75 kDa 105 kDa and 145 kDa respectively. SIK1 SIK2 and SIK3 share structural similarities but differ in their tissue expression. SIK1 is mostly found in the heart and liver SIK2 in adipose tissue and SIK3 shows widespread expression including the brain and skeletal muscle. Mechanically these kinases modify other proteins through phosphorylation influencing cellular activity.
Biological function summary
These kinases regulate various processes essential for maintaining cellular homeostasis. They are not part of any well-known complex but interact with several cellular components. SIKs influence gene expression by inhibiting transcriptional regulators and also regulate metabolism and cell survival. Their action impacts how cells respond to external stimuli largely through modification of transcriptional activity in response to hormonal signals and nutrient availability.
Pathways
SIK1 SIK2 and SIK3 are integral to the AMP-activated protein kinase (AMPK) signaling pathway and the cAMP response element-binding protein (CREB) pathway. These pathways control energy balance and respond to stress conditions. SIKs act alongside AMPK to modulate cellular energy status and also influence the CREB signaling pathway by impacting phosphorylation state. Regulatory relationships exist with proteins like 14-3-3 and HDACs which themselves are key players in cellular metabolism and gene expression.
These kinases have links to metabolic diseases and cancer. SIKs have been associated with obesity due to their role in adipocyte differentiation and lipid metabolism. They also relate to pancreatic cancer where altered kinase activity disrupts normal cell function and proliferation. In cancer SIKs interact with proteins involved in proliferation pathways including mTOR affecting tumor growth and progression.