Abcam, ab272194, Human CG beta ELISA Kit (CGB3)

Size: 1 x 96Tests
Human CG beta ELISA Kit (CGB3) is a single-wash 90-min Simplestep used to quantify Human CG beta (CGB3) with a sensitivity of 2.62 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Urine, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 2.62 pg/mL,
Range:23.44 - 1500 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human CG beta ELISA kit (ab272194) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CG beta protein in serum, plasma and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CG beta with 2.62 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Human Chorionic Gonadotropin (hCG) is a complex glycoprotein composed of two glycosylated subunits alpha and beta which are non-covalently associated and normally produced by placenta during pregnancy. Measurement of intact hCG and of the alpha subunit of hCG appears to give similar results in blood and urine but not the levels of beta subunit. The beta subunits are distinct in each of the hormones and confer receptor and biological specificity. CG beta (CGB3) has an essential role in maternal adaption and stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy. hCG and the free subunits appear not to be useful as serological markers for nontrophoblastic tumors; however, the absolute increase of β-hCG level in choriocarcinoma patients clearly differentiates it from normal pregnancy.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Human chorionic gonadotropin (hCG) is a glycoprotein hormone composed of two subunits alpha and beta. The beta subunit confers its specific biological activity. Its molecular weight ranges around 36.7 kDa. hCG is produced in the placenta during pregnancy and is also expressed in pituitary cells. Known alternatively as the pregnancy hormone hCG plays a critical role in maintaining the luteal function during the early stages of pregnancy.
Biological function summary
HCG regulates several functions related to reproduction. It supports the corpus luteum in ovaries to secrete progesterone during early pregnancy contributing to the uterine lining's maintenance. Moreover hCG can interact with the luteinizing hormone/chorionic gonadotropin receptor (LHCGR) indicating its involvement in broader reproductive functions. Not as well known hCG may exist in various forms such as hCG intact influencing certain biological pathways differently.
Pathways
Scientists study hCG's role in reproductive signaling and gonadotropin pathways. It interacts closely with luteinizing hormone (LH) with substantial overlap in their pathway functions impacting reproductive health and fertility cycles. hCG's activation of specific receptors like LHCGR plays a role in these pathways connecting it to various fertility-related responses in the human body.
HCG levels can be an indicative marker for certain conditions. Elevated hCG levels may signal trophoblastic diseases such as molar pregnancy or gestational trophoblastic neoplasia. Its abnormal expression might also indicate testicular cancer where it acts as a tumor marker. Both conditions relate to hCG through its shared glycoprotein structure with other hormones like thyroid-stimulating hormone (TSH) which links the hormone to broader endocrine disorder research.