Abcam, ab272235, Anti-TCF-4/TCF7L2+TCF-3/TCF7L1 antibody [EPR24701-87]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal TCF-4/TCF7L2 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 3 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24701-87,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The TCF-4/TCF7L2 and TCF-3/TCF7L1 proteins are part of the T-cell factor/lymphoid enhancer-binding factor (TCF/LEF) family. These proteins also known as transcription factor 7 like 2 and transcription factor 7 like 1 function as transcription factors in the Wnt signaling pathway. TCF7L2 and TCF7L1 typically weigh around 65 kDa and 56 kDa respectively. Expression of these proteins occurs in many tissues notably in the colon liver and pancreas. Mechanically they interact with β-catenin to regulate the transcription of Wnt target genes.
Biological function summary
TCF-4/TCF7L2 and TCF-3/TCF7L1 play key roles in controlling gene expression essential for cell proliferation differentiation and embryonic development. These transcription factors often form complexes with β-catenin coactivators or corepressors to modulate gene transcription activity. By influencing genetic programs they contribute to developmental processes and the maintenance of stem cells' properties. Their involvement in embryogenesis highlights their importance in developmental signaling.
Pathways
TCF-4/TCF7L2 and TCF-3/TCF7L1 are significant in the canonical Wnt/β-catenin signaling pathway. This pathway influences many cellular processes including cell fate and cellular proliferation decisions. When Wnt ligands activate the pathway β-catenin accumulates in the cytoplasm and enters the nucleus where it partners with TCF proteins to modify gene expression. This interaction highlights the relationship of TCF proteins with β-catenin and other Wnt-associated elements like Axin and APC which regulate β-catenin stability.
Alterations in TCF7L2 and TCF7L1 activity impact conditions like colorectal cancer and type 2 diabetes. Mutations or misexpression of TCF7L2 show strong links to increased risk of type 2 diabetes across various populations. In colorectal cancer changes in Wnt signaling and TCF's role as transcription factors contribute to oncogenesis. TCF proteins interact with other factors in these contexts such as the adenomatous polyposis coli (APC) protein highlighting their relevance in disease pathology.