Abcam, ab273165, Mouse Syndecan-1 ELISA kit

Size: 1 x 96Tests
Mouse Syndecan-1 ELISA kit is a sandwich ELISA designed to quantify Mouse Syndecan-1 with a sensitivity of 0.35 ng/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 0.5 - 16 ng/mL
Key facts
Sample types:Cell culture supernatant, Other biological fluids, Plasma, Serum,
Reacts with:Mouse,
Sensitivity:< 0.35 ng/mL,
Range:0.5 - 16 ng/mL,
Assay time:2h 45m

Product details:
Mouse Syndecan-1 ELISA kit ab273165 is a sandwich ELISA to measure Mouse Syndecan-1 in serum, plasma, cell culture supernatant, other biological fluids with a sensitivity of 0.35 ng/ml.
How the assay works
A capture antibody highly specific for Syndecan-1 has been coated to the wells of the microtiter strip plate provided during manufacture. Binding of Syndecan-1 in samples and known standards to the capture antibodies and subsequent binding of the biotinylated anti-Syndecan-1 secondary antibody to the analyte is completed during the same incubation period. Any excess unbound analyte and secondary antibody is then removed.The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of Syndecan-1 present in the samples and standards.The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of Syndecan-1 in any sample tested.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Mouse Syndecan-1 ELISA kit ab273165 protocol summary
1. Add 100 μl of sample and diluted standard.
2. Add 50 μl of biotinylated detection antibody. Incubate 2 hours at room temperature.<3> Wash three times then add 100 μl of Streptavidin-HRP.Incubate 30 mins at room temperature.
4.Wash three times then add 100 μl of ready-to-use TMB. Protect from light. Let the color develop for 5-15 mins.
5. Add 100 μl H2SO4 and read absorbance at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Syndecan-1 also known as CD138 is a transmembrane heparan sulfate proteoglycan with a molecular weight of approximately 30 to 80 kDa. The size variation arises from differences in its glycosaminoglycan chains. This protein resides predominantly on the surface of epithelial cells and plasma cells. Researchers commonly use the CD138 marker to identify these cells in biological samples. It plays a significant role in cell-cell and cell-matrix interactions acting as a co-receptor for a variety of growth factors cytokines and extracellular matrix proteins.
Biological function summary
Syndecan-1 participates in tissue repair inflammation and cell signaling ensuring epithelial integrity and communication. Syndecan-1 influences cell behavior by interacting with other components of the extracellular matrix and different membrane receptors. It is also involved in forming and regulating proteoglycan complexes at the cell surface. These complex interactions facilitate the modulation of cell proliferation migration and differentiation which are fundamental to maintaining tissue architecture and function.
Pathways
Syndecan-1 actively contributes to both the Wnt and FGF signaling pathways. Through its interaction with proteins such as miR-15 syndecan-1 modulates cellular responses essential for developmental processes and tissue homeostasis. The connection with these pathways allows syndecan-1 to integrate and relay extracellular signals impacting gene expression and cellular behavior. Its relationship with other proteins like A38 as a co-receptor emphasizes its role in finely tuning signaling cascades within cellular environments.
Syndecan-1 shows a connection to multiple myeloma and breast cancer. The overexpression of syndecan-1 in these conditions disrupts normal cellular functions and promotes tumorigenesis by aiding in the tumor cell's adhesion migration and invasion. Its interaction with proteins such as perCP further demonstrates its role in disease progression providing potential as a therapeutic target. These relationships make syndecan-1 an important focus for research in oncology and other fields of disease study.