Abcam, ab273224, Porcine IL-17F ELISA Kit

Size: 1 x 96Tests
Porcine IL-17F ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Porcine IL-17F in Pig, Rat in Cell Culture Media, Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum,
Reacts with:Rat, Pig,
Assay type:Sandwich (quantitative),
Sensitivity:= 6 pg/mL,
Range:4.1 - 1000 pg/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Porcine IL-17F ELISA Kit (ab273224) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Porcine IL-17F in serum, plasma (Collect plasma using EDTA and citrate as an anti-coagulant.
Heparin is not recommended
as anti-coagulant for use in this assay
) and cell culture supernatants.
This assay employs an antibody specific for Porcine IL-17F coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17F present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Porcine IL-17F antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17F bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C