Abcam, ab273278, Phospholipase A2 Activity Assay Kit (Fluorometric)

Size: 100Test
Phospholipase A2 Activity Assay Kit (Fluorometric) (ab273268) provides a quick, sensitive and simple way for measuring PLA2 activity in various biological samples. Readout on any fluorometric plate reader (Ex/Em 388/513 nm). Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
Detection method:Fluorescent,
Sample types:Tissue, Suspension cells, Adherent cells,
Assay type:Enzyme activity (quantitative),
Assay Platform:Microplate

Product details:
Phospholipase A2 Activity Assay Kit (Fluorometric) (
ab273268
) provides a quick, sensitive and simple way for measuring PLA2 activity in various biological samples.
Phospholipase A2 assay principle
In this assay, active PLA2 cleaves a synthetic thiophospholipid, producing a lysothiophospholipid which reacts with a fluorogenic probe to produce a highly fluorescent product detectable in the visible range (Ex/Em= 388/513 nm).
The synthetic thiophospholipid used is 1,2-bis(heptanoylthio) Glycerophosphocholine, which is a substrate for all phospholipase A2s (PLA2s) with the exception of cPLA2 and PAF-acetyl hydrolase.
The assay is simple to perform, high-throughput adaptable and can detect less than 0.1 mU of PLA2 activity.
Related PLA2 assay kits
For a colorimetric PLA2 assay usePhospholipase A2 Activity Assay Kit
ab133089
To detect cytosolic PLA2, use Cytosolic Phospholipase A2 Assay Kit
ab133090
Other notes
This product was previously called K400 Phospholipase A2 Activity Assay Kit (Fluorometric). Biovision was acquired by Abcam in 2021.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Phospholipase A2 (PLA2) is an enzyme that hydrolyzes the sn-2 ester bond of phospholipids releasing fatty acids and lysophospholipids. This process is essential for producing pro-inflammatory eicosanoids and other lipid mediators. Commonly known as PLA2 or phospholipase its molecular mass varies depending on its type ranging from 14 to 45 kDa. PLA2 is expressed in many tissues including the pancreas brain and immune cells highlighting its diverse role in different physiological contexts.
Biological function summary
Phospholipase A2 contributes to membrane remodeling and the release of arachidonic acid a precursor to prostaglandins and leukotrienes vital for inflammatory responses. It often functions as part of a complex with other proteins that regulate its activity ensuring precise lipid signaling and homeostasis. Understanding PLA2 function leads to insights into cell signaling and inflammatory regulation.
Pathways
Phospholipase A2 plays a significant role in the arachidonic acid pathway and is involved in the production of eicosanoids. These pathways are important for the inflammatory response and involve interactions with cyclooxygenases (COX) and lipoxygenases (LOX). Through these pathways PLA2 activity can influence immune responses and cellular metabolism making it an important component in inflammatory signaling cascades.
Phospholipase A2 is implicated in conditions like arthritis and atherosclerosis. Its role in inflammation makes it a potential target for therapeutic interventions. PLA2 interacts with proteins such as COX-2 which are involved in the inflammatory response in arthritis while its role in lipid metabolism links it to the formation of atherosclerotic plaques. Understanding these connections can aid in developing treatments for inflammatory and cardiovascular diseases.