Abcam, ab273435, Anti-SARS-CoV-2 orf7a antibody [3C9]

Size: 100µL
Mouse Monoclonal NS7A antibody. Carrier free. Suitable for WB, ICC/IF and reacts with SARS-CoV-2 samples.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:3C9,
Isotype:IgG1,
Carrier free:Yes,
Reacts with:SARS-CoV-2,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The ORF7a protein also known as accessory protein 7a acts as an important virulence factor in SARS-CoV-2 the virus causing COVID-19. The protein has a molecular mass of approximately 12 kDa and is expressed in the endoplasmic reticulum. It extends into the lumen where it can bind to proteins and other molecules. ORF7a is known to interact with different host proteins manipulating cellular machinery to aid viral replication and pathogenesis.
Biological function summary
Functions of the ORF7a protein extend to immune system inhibition. It can interfere with type I interferon signaling reducing the host's antiviral response. As part of viral assembly ORF7a contributes to the efficient packaging and release of viral particles. It does not form a complex with other viral proteins but acts independently to execute its functions within the infected host cell.
Pathways
Roles of the ORF7a protein integrate into immune escape and virion production processes. It participates in immune evasion pathways by inhibiting signaling pathways that interfere with interferon production like the JAK-STAT pathway. Additionally it has associations with other SARS-CoV-2 proteins like ORF3b which jointly compromise host cell functions and enhance virus survival.
The ORF7a protein has strong connections to the manifestation of COVID-19-related symptoms. The inhibition of interferon signaling contributes to respiratory distress observed in severe cases of COVID-19. Studies also indicate links to the inflammatory responses involving other viral proteins such as the N protein which together can exacerbate the severity of SARS-CoV-2 infections.