Abcam, ab273436, Anti-SARS spike glycoprotein S1 antibody [7G12]

Size: 100µL
Mouse Monoclonal SPIKE antibody. Suitable for IP, WB and reacts with Transfected cell lysate - Human, SARS-CoV samples.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:7G12,
Isotype:IgG1,
Carrier free:No,
Reacts with:SARS-CoV,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:This antibody detects SARS-CoV spike protein (S1 subunit), and may have minor cross-reactivity with SARS-CoV-2 spike protein. No cross-reactivity with MERS-CoV spike protein is expected.

Product details:
Applications overview
Tick: Tested and Guaranteed to work X: Will not work —: No data

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified from tissue culture supernatant., Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The SARS spike glycoprotein S1 also known as Spike S1 is a structural protein of the SARS coronavirus. This glycoprotein serves as the major determinant of host specificity and immune evasion mediating virus entry into host cells. Its function involves binding to the angiotensin-converting enzyme 2 (ACE2) receptor on host cells. The Spike S1 component has an approximate molecular mass of 90 kDa and expresses heavily on the viral surface facilitating the attachment of the virus to the host cell membrane.
Biological function summary
The SARS spike glycoprotein S1 plays an important role in the viral entry process. It is part of the larger spike protein complex which consists of S1 and S2 subunits. The S1 subunit binds directly to the host ACE2 receptor initiating conformational changes that allow the S2 subunit to promote membrane fusion. This protein complex is critical for the fusion of the viral and cellular membranes a necessary step for viral entry and infection.
Pathways
The interaction of the S1 glycoprotein with the host ACE2 receptor activates the renin-angiotensin system pathway. This protein-receptor binding influences the downregulation of ACE2 and contributes to the pathogenesis of SARS. Additionally the Spike S1 protein influences inflammatory responses through its interaction with Toll-like receptors. This interaction triggers immune responses impacting related proteins such as pro-inflammatory cytokines and chemokines.
The SARS spike glycoprotein S1 is closely associated with the pathogenesis of COVID-19. Its binding to ACE2 can lead to dysregulation of blood pressure and inflammatory responses contributing to severe respiratory syndrome. The Spike S1 protein also plays a role in the potential development of myocarditis a complication of COVID-19 where it may induce cardiac inflammation. Connections with other viral proteins including the nucleocapsid protein further implicate the Spike S1 in enhancing viral assembly and pathogenicity during infection.