Abcam, ab273438, Anti-CRISPR-associated endonuclease Cas12a antibody [3D3-F7]

Size: 20µg / 100µg / 1mg
Mouse Recombinant Monoclonal CRISPR-associated endonuclease Cas12a antibody. Suitable for WB and reacts with Transfected cell lysate - Acidaminococcus sp. samples.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:3D3-F7,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Acidaminococcus sp.,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Reacts with Acidaminococcus sp.

Product details:
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CRISPR-associated endonuclease Cas12a also known as Cpf1 is a single-effector protein used in genome editing. This protein has a molecular weight around 150 kDa and is found in various bacterial species as a defense mechanism against viral infections. Cas12a performs its function by creating double-strand breaks in DNA through its endonuclease activity. It recognizes specific DNA sequences via a guide RNA and cleaves the DNA allowing for precise genetic modifications.
Biological function summary
Cas12a serves an important role in the CRISPR-Cas adaptive immune system in bacteria. It is part of the Class 2 CRISPR systems and functions in a complex with a single guide RNA (sgRNA). In this complex Cas12a distinguishes itself from other CRISPR-associated proteins by requiring a simpler PAM sequence which offers greater flexibility in target DNA recognition. Cas12a's ability to cleave both strands of DNA with precision makes it a valuable tool for genetic engineering and functional genomics studies.
Pathways
Cas12a enables targeted edits in genetic pathways contributing to advancements in synthetic biology and gene therapy applications. Cas12a interacts with the DNA repair machinery influencing pathways like non-homologous end joining (NHEJ) and homology-directed repair (HDR). These pathways are also influenced by proteins such as DNA ligase IV in NHEJ and RAD51 in HDR showcasing how Cas12a-mediated DNA modifications are repaired and integrated.
Cas12a has potential applications in treating genetic disorders through targeted gene correction or disruption. Cas12a can contribute to research and therapeutic strategies for diseases like sickle cell anemia and muscular dystrophy where precise genome editing can address genetic defects. This system related to human proteins involved in DNA repair pathways may offer innovative solutions in correcting disease-causing mutations and restoring normal cellular functions.