Abcam, ab273463, Anti-PspCas13b antibody [12F2-A10] - BSA and Azide free

Size: 100µg / 1mg
Mouse Recombinant Monoclonal PspCas13b antibody. Carrier free. Suitable for WB and reacts with Transfected cell lysate - Prevotella pectinovora samples.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:12F2-A10,
Isotype:IgG2a,
Carrier free:Yes,
Reacts with:Prevotella pectinovora,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Reacts with Prevotella sp. P5, 125

Product details:
ab273463 is the carrier-free version of
ab273440
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PspCas13b also known as CRISPR-associated protein 13b is an RNA-guided ribonuclease found in the CRISPR-Cas system of the bacterium Prevotella sp. P5-125. The protein consists of 1291 amino acids giving it a molecular mass of approximately 140 kDa. Researchers have discovered it primarily in bacterial species where it acts as a part of the bacterial immune system against phages. PspCas13b stands out because it exhibits programmable RNA-targeting activity making it effective at recognizing and cleaving viral RNA.
Biological function summary
PspCas13b operates by binding to CRISPR RNA (crRNA) to form an active complex. This complex targets single-stranded RNA with high specificity due to its RNA-guided endonuclease activity. Unlike many other CRISPR proteins PspCas13b does not cut DNA which prevents unwanted off-target DNA cleavage. The protein's RNA-targeting activity makes it a valuable tool for degrading specific RNA sequences in research and it functions independently without forming large multisubunit complexes.
Pathways
PspCas13b fits well into the adaptive immune responses in bacteria where it acts as part of a defense mechanism against RNA viruses and mobile genetic elements. It shows similarities with other RNA-targeting proteins like Cas9 though Cas9 targets DNA instead. Additionally PspCas13b's action is closely aligned with the ribonucleoprotein complexes where crRNA processing proteins initiate the CRISPR immune response allowing precise RNA interference.
Scientists explore PspCas13b’s potential in antiviral therapies and RNA-related diseases given its unique RNA-targeting capacity. There is evidence linking PspCas13b to therapeutic applications in viral infections by identifying and degrading viral RNA without affecting host DNA. Moreover it links to RNA-binding proteins involved in genetic disorders where RNA misregulation occurs. These features highlight the importance of PspCas13b in the development of innovative treatments targeting RNA-based illnesses.