Abcam, ab273709, Human NUB1 knockout HeLa cell line

Size: 1000000Cells / vial
NUB1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by CRISPR/Cas9; X = 5 bp deletion; Frameshift: 100%. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Mutation description:Knockout achieved by CRISPR/Cas9; X = 5 bp deletion; Frameshift: 100%,
Disease:Adenocarcinoma

Product details:
Recommended control:
Human wild-type HeLa cell line (ab271142). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NUB1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NUB1 also known as NEDD8 ultimate buster-1 functions as a negative regulator of NEDD8 conjugation. This protein weighs around 65 kDa. Researchers find NUB1 to be widely expressed in the human body with higher expression levels observed in organs like the heart and brain. NUB1 interacts mainly with ubiquitin-like proteins including NEDD8 and facilitates their degradation by the proteasome reducing NEDD8's presence in cells.
Biological function summary
NUB1 influences protein homeostasis and signal transduction. It associates with the proteasome to target and eliminate neddylated proteins contributing to the regulation of protein turnover. NUB1 also forms part of a complex that affects the cellular levels of specific proteins through the ubiquitin-proteasome system. This action helps maintain cellular protein balance and impacts various cellular functions.
Pathways
The ubiquitin-proteasome system revolves around NUB1 as it regulates neddylation and ubiquitination processes. NUB1 connects to the neddylation pathway which includes proteins like NEDD8 and UBA3 influencing protein activities and degradation. Through its association with ubiquitination it regulates the stability and function of key cellular proteins ensuring proper cell function and preventing harmful protein accumulation.
NUB1 shows a connection with neurodegenerative diseases and certain cancers. In neurodegenerative conditions like Alzheimer's disease NUB1's regulatory function impacts protein aggregates' formation potentially influencing disease progression. NUB1 also interacts with proteins linked to cancer pathways such as ubiquitin implicating it in the development of certain malignancies. Adequate function and regulation of NUB1 may therefore play a role in mitigating these diseases.