Abcam, ab273720, Human PSENEN (PEN2) knockout HEK-293 cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
PSENEN KO cell line available to order. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control available. Knockout achieved by CRISPR/Cas9; X = 1 bp deletion, 1 bp insertion; Frameshift: 100%. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Next Generation Sequencing,Western blot,
Mutation description:Knockout achieved by CRISPR/Cas9; X = 1 bp deletion, 1 bp insertion; Frameshift: 100%

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PSENEN, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PEN2 also known as Presenilin Enhancer 2 is a component of the gamma-secretase complex. It has a molecular mass of approximately 12 kDa. PEN2 is expressed in a variety of tissues including neuronal cells and across several cell lines like the HEK 293. This protein plays an essential mechanical role in stabilizing and activating the gamma-secretase complex which is necessary for its protease activity.
Biological function summary
PEN2 is an important element of the gamma-secretase complex composed of presenilin nicastrin APH-1 and PEN2. Its role involves the facilitation of proper maturation and assembly of the complex contributing to the proteolytic processing of several type I transmembrane proteins including the amyloid precursor protein (APP) and Notch. PEN2 plays a part in these cellular processes by ensuring that the gamma-secretase complex functions correctly impacting cellular signaling and communication.
Pathways
Gamma-secretase activity where PEN2 is involved is central to the Notch signaling pathway and the amyloidogenic pathway. The Notch signaling pathway is fundamental in cell differentiation proliferation and apoptosis and relies significantly on the function of the gamma-secretase complex. The amyloidogenic pathway involves the processing of APP to produce amyloid-beta peptides which PEN2's role in the gamma-secretase complex influences. Related proteins in these pathways include presenilin which is an active component in Notch and APP cleavage.
Mutations or dysregulation of PEN2 can have links to Alzheimer's disease and certain cancers. Alzheimer's disease is associated with the production of amyloid-beta peptides which accumulate into plaques a process influenced by PEN2 through gamma-secretase activity with APP. Dysregulated Notch signaling where PEN2 is involved is linked to various cancers due to its role in controlling cell fate decisions. PEN2's interactions with proteins like presenilin and APP highlight its potential impact on these conditions.