Abcam, ab273753, Human PHF23 knockout HEK-293 cell line

Size: 1000000Cells / vial / 2 x 1000000Cells / vial
PHF23 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control available. Knockout achieved by CRISPR/Cas9; X = 8 bp deletion; Frameshift: 99%. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Next Generation Sequencing,
Mutation description:Knockout achieved by CRISPR/Cas9; X = 8 bp deletion; Frameshift: 99%

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PHF23, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions--196°C, -80°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PHF23 also known as Plant Homeodomain Finger 23 is a protein involved in chromatin dynamics and regulation of gene expression. It has a molecular mass of approximately 43 kDa. PHF23 contains a zinc finger domain which is important for its role in binding to histones. The protein expresses in various tissues but shows higher expression levels in certain organs like the brain and testes. Its expression pattern suggests an involvement in critical regulatory processes.
Biological function summary
PHF23 plays a role in the modification of chromatin structure influencing gene transcription. The protein participates in the formation of a complex that is involved in transcriptional repression. It particularly associates with components of the nucleosome acting as a mediator in processes that alter the accessibility of DNA for transcription factors and enzymes. The protein's ability to bind to chromatin allows it to directly impact the epigenetic state of cells.
Pathways
PHF23 is a part of the chromatin remodeling pathway and is involved in the regulation of gene transcription. Its interaction with methylated histones connects it to the pathway of histone modification. It works alongside other proteins like LSD1 which is a lysine-specific demethylase and this collaborative function influences the transcriptional output of the cell. The protein's actions contribute to maintaining the balance between gene activation and repression which is essential for normal cellular functions.
PHF23 relates to conditions involving abnormal chromatin remodeling such as certain cancers. Its alteration affects the typical transcriptional control which can lead to tumorigenesis. The protein interacts with other proteins including members of the histone demethylase family like KDM1A influencing pathways that become dysregulated in cancerous states. Studies suggest that understanding PHF23's exact role offers potential therapeutic targets for diseases where chromatin misregulation plays an important role.