Abcam, ab274329, Anti-KMT3A / HYPB / HIF-1 antibody [EPR20927-67] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal KMT3A / HYPB / HIF-1 antibody. Carrier free. Suitable for WB and reacts with Mouse, Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR20927-67,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab274329 is the carrier-free version of
ab239350
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
KMT3A also known as HYPB (Huntington's disease gene-related protein B) or HIF-1 is a histone methyltransferase enzyme that mechanically transfers methyl groups to histone H3 at lysine 4 and lysine 36. This activity important for chromatin modification influences transcriptional regulation. The molecular mass of KMT3A is approximately 130 kDa. This enzyme is expressed in various tissues such as the brain heart and skeletal muscle highlighting its extensive physiological role.
Biological function summary
KMT3A modifies chromatin structure to regulate gene expression. Its histone methylation function plays a critical role in the modulation of transcriptional activity ensuring that genes are turned on or off when necessary for cellular function. Additionally KMT3A forms part of complex protein networks that mediate gene expression changes in response to cellular signals. The precise regulation of this activity is vital for maintaining normal cellular function and responding to environmental cues.
Pathways
KMT3A engages in significant pathways such as the transcriptional regulation and epigenetic modification pathways. It interacts with various proteins including CoREST and LSD1 within these pathways to modulate gene expression and influence cellular differentiation. Through these interactions KMT3A ensures the correct genetic programs are executed aligning with developmental and homeostatic requirements.
KMT3A has been implicated in neurological conditions such as Huntington's disease. The abnormal regulation or mutation of this target can disrupt its activity and lead to improper chromatin modification which results in altered gene expression and contributes to disease progression. Furthermore KMT3A's interaction with the HIF-1α pathway also links it to cancer as dysregulation can aid in tumor growth and progression by affecting gene expression patterns associated with cell proliferation and survival.