Abcam, ab275389, Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal KAT3B / p300 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Rat, Human, Recombinant full length protein - Human, Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23495-276,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, ICC/IF, Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab275389 is the carrier-free version of
ab275379
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The CREBBP and p300 also known as KAT3B are essential transcriptional coactivators. Mechanically they function as acetyltransferases adding acetyl groups to histones which leads to the relaxation of chromatin structure and the activation of gene transcription. This process is critical for transcription regulation in eukaryotic cells. CREBBP has a molecular weight of approximately 265 kDa and is expressed in various tissues throughout the body including the heart lung and brain. Both of them possess bromodomain functions important for recognizing acetyl-lysine residues.
Biological function summary
CREBBP and p300 operate by interacting with a wide array of transcription factors and serve as scaffold proteins. They facilitate the assembly of large multiprotein complexes necessary for efficient initiation elongation or termination of transcription. Through acetylation these proteins modulate the activity of over 400 target proteins influencing diverse cellular processes like cell growth differentiation and repair. As part of the transcriptional activation complex CREBBP and p300 also acetylate non-histone proteins affecting their stability and function.
Pathways
CREBBP and p300 play pivotal roles in pathways such as the Notch signaling and the cAMP response element-binding protein (CREB) pathway. In the CREB pathway they interact with CREB to enhance the transcription of genes essential for neuronal activity and plasticity. In the Notch signaling pathway they contribute to cell fate decisions in developmental processes. These proteins collaborate with other histone acetyltransferases to maintain the dynamic regulation of gene expression.
Defects or mutations in CREBBP and p300 associate with Rubinstein-Taybi syndrome (RSTS) and certain cancers such as gastric cancer. In RSTS haploinsufficiency of the protein results in a range of developmental abnormalities due to impaired transcriptional regulation. In cancers abnormal acetyltransferase activity alters cell cycle regulation and apoptosis contributing to tumorigenesis. Their interaction with tumor suppressors and oncogenes highlights their integral role in maintaining cellular homeostasis and disease prevention.