Abcam, ab275752, Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free

Size: 100µg / 1mg
Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation. Suitable for Western Blot, Flow Cytometry, IHC-P, IHC-Fr, ICC/IF in Human, Mouse, Rat . - KO validated for confirmed specificity - BSA, sodium azide, and glycerol-free for easy conjugation - Biophysical QC for unrivalled batch-batch consistency
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23630-49,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, ICC/IF, IHC-Fr, Flow Cyt (Intra), IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of SCA2?
Anti-SCA2 [EPR23630-49] - BSA and Azide free (ab275752) specifically detects a band for SCA2 (UniProt: Q99700) at a molecular weight of 140kDa.
Specificity confirmed
The specificity of Anti-SCA2 antibody [EPR23630-49] - BSA and Azide free (ab275752) has been confirmed by Western blot testing in ATXN2 Knockout HAP1 cells.
Other related products
We have a range of other formats of antibody clone [EPR23630-49] also available for your convenience:
ab254362
, Carrier free - ab275752, Alexa Fluor® 647 -
ab286816
, Alexa Fluor® 488 -
ab300055
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SCA2 also known as Spinocerebellar Ataxia Type 2 refers to a protein primarily affected by mutations in the ATXN2 gene. It weighs around 150 kDa. SCA2 expresses predominantly in the brain with significant presence in Purkinje cells of the cerebellum as well as regions like the thalamus and hippocampus. It functions as an essential component within neuron-specific structures and may associate with RNA processing events.
Biological function summary
SCA2 shows significant interactions within the neuronal RNA processing machinery. This protein is part of a large complex involved in regulating RNA stability and translation. Its role contributes significantly to maintaining neuron function and viability affecting synaptic signaling and plasticity which are critical for cognitive processes and motor control. SCA2 influences neural communication through modulation and degradation of messenger RNAs.
Pathways
SCA2 plays a role in the mRNA metabolic process related cellular pathways. It interacts in pathways like RNA transport and degradation linking it functionally to proteins such as TDP-43 and FUS which also have prominent roles in RNA processing. These associations support communication across neurons and ensure accurate protein synthesis necessary for neuronal health and function.
Malfunction or mutation in SCA2 relates directly to spinocerebellar ataxia type 2 a degenerative disorder affecting movement. The imbalance in related pathways often leads to pathological changes contributing to dysfunctions in Ataxin-2 often seen alongside interactions with proteins like Huntingtin which elucidate its involvement in neurological diseases. Such connections imply a substantial impact on neuronal survival and disease progression.