Abcam, ab275782, Anti-Neurogranin antibody [Ng2] - BSA and Azide free (Detector)

Size: 100µg / 1mg
Mouse Recombinant Monoclonal Neurogranin antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:Ng2,
Isotype:IgG2b,
Carrier free:Yes,
Reacts with:Human,
Applications:sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab275782 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Neurogranin. The recommended pair for sandwich ELISA is:
Capture:
ab275781
, Human Neurogranin Capture Antibody (unconjugated)
Detector: ab275782, Human Neurogranin Detector Antibody (unconjugated)
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Neurogranin also known as NRGN is a small neuronal protein with an approximate molecular mass of 7.6 kDa. It is mainly expressed in the brain predominantly within the dendrites of neurons. Neurogranin binds to calmodulin in the presence of calcium playing an important role in synaptic plasticity. This protein acts as a kinase substrate influencing signal transduction pathways tied to learning and memory. Due to its specific expression pattern neurogranin serves as a significant molecular marker for studying the nervous system.
Biological function summary
Neurogranin functions as a modulator in cerebellar signaling and synaptic transmission. It influences calcium-calmodulin signaling facilitating the activation of proteins important in the central nervous system. Neurogranin does not form part of a multi-protein complex but interacts closely with calmodulin to regulate downstream signal cascades. These interactions emphasize its fundamental role in modulating processes that influence cognitive functions like learning and memory.
Pathways
Neurogranin significantly impacts the calcium signaling pathway and contributes to the regulation of long-term potentiation. It closely associates with proteins such as Protein Kinase C (PKC) and calmodulin-dependent protein kinases which are instrumental in these pathways. By modulating these essential signaling routes neurogranin affects intracellular signals that are vital for synaptic plasticity and enhanced neural communication.
Neurogranin has a noted involvement in conditions like Alzheimer's disease and cognitive impairment. Changes in neurogranin expression levels are linked to the progression of Alzheimer's where reduced levels may contribute to the disruption of synaptic plasticity. Interactions with amyloid-beta peptides and other proteins such as tau highlight its potential as a biomarker for neurodegenerative diseases. These connections underline neurogranin's significant role in understanding cognitive-related disorders and guiding therapeutic research.