Abcam, ab277165, Human TUBB2B knockout HEK-293 cell line

Size: 1000000Cells / vial / 2 x 1000000Cells / vial
TUBB2B KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control available. Knockout achieved by CRISPR/Cas9 X = 1 bp deletion Frameshift: 100%. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Next Generation Sequencing,
Mutation description:Knockout achieved by CRISPR/Cas9 X = 1 bp deletion Frameshift: 100%

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TUBB2B, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions--196°C, -80°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TUBB2B also known as Tubulin beta-2B chain is a member of the beta tubulin family playing an integral role in the formation of microtubules. It has a molecular weight of approximately 50 kDa. This protein is expressed extensively in the brain and its expression is critical during the development of the nervous system. TUBB2B participates in the intricate assembly of the microtubule network which provides structural support and facilitates intracellular transport.
Biological function summary
Tubulin beta-2B chain contributes to processes that are essential for neurodevelopment. This protein forms part of the microtubule complex which is involved in the dynamics and stability of the cytoskeleton. Alongside other tubulins TUBB2B is necessary for axonal growth guidance and synaptic transmission. It plays an important role in mitosis where it ensures proper chromosome alignment and segregation by forming the mitotic spindle.
Pathways
TUBB2B participates in the microtubule polymerization pathway which is pivotal for cell division and differentiation. This protein is a part of the network involving proteins like MAP2 and Tau which stabilize microtubules. It also functions within the neurodevelopmental pathways interacting with motor proteins such as dynein and kinesin to govern neuronal migration and positioning vital processes for brain development.
Mutations in TUBB2B have links to cortical dysplasia a neurological disorder that affects brain structure. It's associated with malformations like lissencephaly and polymicrogyria conditions characterized by abnormal brain morphology due to impaired neuronal migration. The protein interacts with DCX (doublecortin) which further elucidates its involvement in cortical development. These mutations can also contribute to neurodevelopmental disorders that manifest as epilepsy highlighting the importance of TUBB2B in maintaining normal brain function.