Abcam, ab277389, Rabbit IL-6 ELISA Kit

Size: 1 x 96Tests
Rabbit IL-6 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rabbit IL-6 in Rabbit in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Rabbit,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.4 pg/mL,
Range:0.4 - 100 pg/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Rabbit IL-6 ELISA Kit (ab277389) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rabbit IL-6 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Rabbit IL-6 coated on a 96-well plate. Standards and samples are pipetted into the wells and Rabbit IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Rabbit IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Rabbit IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Interleukin-6 (IL-6) a cytokine also known as IFN-beta2 plays a significant role in immune response and inflammation. The IL-6 protein has a molecular weight of approximately 20-26 kDa. Expression of IL-6 occurs in various cell types including T cells macrophages and fibroblasts. Researchers often measure IL-6 levels in biological samples using IL-6 ELISA kits an essential tool for studying this protein’s function and presence in experimental and clinical settings.
Biological function summary
IL-6 influences immune regulation and acts as part of the acute phase response. It stimulates the production of acute-phase proteins and supports the differentiation of B cells into antibody-producing cells. IL-6 is not known to be part of a larger complex acting primarily as a single entity in signal transduction. Moreover IL-6 impacts the metabolism of iron and bone homeostasis showing its multifunctional nature.
Pathways
IL-6 forms an integral part of several signaling routes particularly the JAK-STAT pathway. In this context IL-6 interacts with signal transducer proteins like STAT3 to transmit signals from the cell surface to the nucleus affecting gene expression. Another important pathway is the MAPK pathway through which IL-6 influences cell proliferation and survival. These interactions reflect IL-6's diverse effects in cellular processes.
IL-6's association with rheumatoid arthritis and multiple myeloma emphasizes its role in chronic inflammation and cancer. In rheumatoid arthritis IL-6 contributes to inflammation and joint damage often together with TNF-alpha highlighting a potential target for anti-inflammatory therapies. In multiple myeloma IL-6 supports the survival and proliferation of cancerous plasma cells highlighting its importance in cancer progression and possible treatment targets.