Abcam, ab277390, Mouse Insulin ELISA Kit

Size: 1 x 96Tests
Mouse Insulin ELISA kit is a sandwich ELISA designed to quantify both native and recombinant mouse insulin (INS) with a sensitivity of 5 µIU/mL. - Wide dynamic range – quantifies 6.25–400 µIU/mL - Broad sample compatibility – works with serum, plasma (citrate, EDTA), and cell culture supernatants - Assay platform – pre-coated microplate (12 x 8-well strips) for easy setup and reliable results - Cited in over 15 publications
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 5 µlU/mL,
Range:6.25 - 400 µlU/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse Insulin ELISA Kit ab277390 is a sandwich ELISA kit to measure mouse insulin (INS) with a sensitivity of 5 µIU/mL.
How the assay works
Mouse Insulin ELISA Kit ab277390 uses a pair of antibodies each specific to different epitopes at the target of interest: the capture antibody is coated on the surface of the multi-well plate to facilitate the immobilization of the antigen. The other antibody is conjugated and facilitates the detection of the antigen.
Assay specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility.
Mouse Insulin ELISA Kit ab277390 protocol summary
Add standards or samples to the wells, incubate 2 hrs
Wash, then add 50 µl of Biotinylated Antibody per well. Incubate 1 hr
Wash, then add 50 µl of SP Conjugate per well. Incubate 30 min
Wash, then add 50 µl of Chromogen Substrate per well. Incubate 20 min
Add 50 µl of Stop Solution per well. Read at 450 nm immediately
How other researchers are using Mouse Insulin ELISA Kit ab277390
Mouse Insulin ELISA Kit ab277390 has been used in publications to study the mechanisms of sulfosuccinimidyl oleate in fatty acid transport as a potential treatment for obesity
and the mechanisms of hypothalamic neuron regulation on bone homeostasis
References:
Xiaoping Liu et al. 20223 PMID: 37305546,
Eiichi Hinoi et al. 2023 PMID: 36862514
Related and recommended products
Mouse Insulin ELISA Kit ab277390 is commonly used to study diabetes and insulin disorders.
Other kits for measuring metabolic health include:
- Mouse Albumin ELISA Kit
ab207620
- Mouse Leptin ELISA Kit
ab199082
- Mouse Adiponectin ELISA Kit
ab226900
Mouse Insulin ELISA Kit ab277390 is a sandwich ELISA to measure mouse Insulin (mouse ins) in serum, plasma, and cell culture supernatant with a sensitivity of 5 µIU/mL.
How the assay works
This assay employs an antibody specific for Mouse Insulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Mouse Insulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Mouse Insulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Mouse Insulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Assay specificity
This assay detects mouse, human, rat, porcine and bovine Insulin.
Mouse Insulin ELISA Kit ab277390 protocol summary
1. Add samples and standards to wells. Incubate at room temperature.
2. Wash each well and add biotinylated antibody. Incubate at room temperature.
3. Wash each well and add Streptavidin Solution. Incubate at room temperature.
4. Add TMB Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read immediately.
How other researchers are using Mouse Insulin ELISA Kit ab277390
Mouse Insulin ELISA Kit ab277390 has been used in publications to study the mechanisms of sulfosuccinimidyl oleate in fatty acid transport as a potential treatment for obesity
and the mechanisms of hypothalamic neuron regulation on bone homeostasis
References:
Xiaoping Liu et al. 20223 PMID: 37305546,
Eiichi Hinoi et al. 2023 PMID: 36862514
Related and recommended products
Mouse Insulin ELISA Kit ab277390 is commonly used to study diabetes and insulin disorders.
Other kits for measuring metabolic health include:
- Mouse Albumin ELISA Kit
ab207620
- Mouse Leptin ELISA Kit
ab199082
- Mouse Adiponectin ELISA Kit
ab226900

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Insulin a peptide hormone plays an essential mechanical role in carbohydrate and fat metabolism. Insulin alternatively known as bovine insulin in some research contexts has a molecular mass of around 5800 Da. Beta cells in the pancreas express insulin where it gets secreted directly into the bloodstream. The hormone regulates glucose levels by facilitating the uptake of glucose into cells such as muscle and adipose tissues.
Biological function summary
Insulin influences several key metabolic processes by binding to its receptor a component of the insulin receptor substrate family. This triggers a cascade of events within cells promoting the uptake of glucose and converting it into energy. Insulin operates as a singular entity but plays a critical role in forming a complex with its receptor. This interaction triggers downstream effects that alter the activity of enzymes and transcription factors involved in glucose and lipid metabolism.
Pathways
Insulin is critical for the regulation of the PI3K-AKT signaling pathway and the MAPK pathway. These pathways modulate processes such as cell growth proliferation and survival. Insulin interacts closely with other proteins in these pathways such as the insulin receptor substrate proteins and influences downstream proteins like AKT kinase which has a significant role in mediating the metabolic effects initiated by insulin binding.
Insulin is most notably linked to diabetes mellitus characterized by insufficient insulin production or action. This deficiency disrupts normal glucose homeostasis leading to hyperglycemia. Other associated proteins include glucagon which counteracts insulin's role in lowering blood glucose levels and IRS-1 an important element through which insulin signaling is transduced. Researchers utilize mouse insulin ELISA and insulin ELISA kits to better understand these relationships and develop treatments for insulin-related disorders.