Abcam, ab277415, Human Superoxide Dismutase 3/EC-SOD ELISA Kit

Size: 1 x 96Tests
Human Superoxide Dismutase 3/EC-SOD ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Superoxide Dismutase 3/EC-SOD in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.65 ng/mL,
Range:0.61 - 150 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Superoxide Dismutase 3/EC-SOD ELISA Kit (ab277415) is an
in-vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human Superoxide Dismutase 3/EC-SOD in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Superoxide Dismutase 3/EC-SOD coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Superoxide Dismutase 3/EC-SOD present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Superoxide Dismutase 3/EC-SOD antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Superoxide Dismutase 3/EC-SOD bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Superoxide Dismutase 3 also known as EC-SOD is an antioxidant enzyme with a molecular weight of approximately 135 kDa. It's an extracellular enzyme that catalyzes the dismutation of superoxide radicals into hydrogen peroxide and molecular oxygen. EC-SOD is found mostly in the extracellular matrix tissues such as lungs blood vessels and heart. Its activity depends on the presence of metal ions copper and zinc leading its classification as a Cu/Zn SOD enzyme. Scientists often study EC-SOD using ELISA assays because of its importance in neutralizing reactive oxygen species.
Biological function summary
The enzyme acts as an important regulator of oxidative stress by reducing levels of superoxide radicals. EC-SOD forms a complex with heparan sulfate proteoglycans in the extracellular matrix which influences its stability and distribution. This enzyme plays a role in maintaining the redox balance influencing cell signaling inflammation and tissue damage which makes it important in managing oxidative stress in different tissues including the cardiovascular and respiratory systems.
Pathways
Superoxide dismutase 3 participates in antioxidant pathways important for cellular defense against oxidative stress. It is particularly important in the oxidative stress response pathway. By reducing superoxide radicals EC-SOD influences pathways involving other antioxidant proteins like catalase and glutathione peroxidase. These interactions help prevent the accumulation of harmful oxidants and maintain cellular homeostasis. EC-SOD's action connects closely with other superoxide dismutases such as Cu/Zn SOD and MnSOD within these protective pathways.
Enhanced or impaired EC-SOD function associates with various health issues such as cardiovascular diseases and chronic obstructive pulmonary disease (COPD). EC-SOD has been shown to protect vascular health by reducing oxidative stress related damages which can mitigate the risk of atherosclerosis. In the context of respiratory conditions like COPD alterations in EC-SOD expression can disrupt lung tissue function and inflammatory responses. Its interactions with proteins like catalase further contribute to its protective role against cell and tissue damage during disease progression.