Abcam, ab277428, Human GOLPH2 ELISA Kit

Size: 1 x 96Tests
Human GOLPH2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human GOLPH2 in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.5 ng/mL,
Range:0.573 - 700 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human GOLPH2 ELISA Kit (ab277428) is an
in-vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human GOLPH2 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human GOLPH2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human GOLPH2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human GOLPH2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human GOLPH2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GOLPH2 also known as GOLM1 or Golgi membrane protein 1 has a significant mechanical role in the cell. It is a 73-kilodalton protein found mainly in the Golgi apparatus. This protein gets expressed in various tissues with notable levels in the liver and other organs involved in secretion. It acts as a transmembrane protein in the Golgi where it helps in processing and sorting secretory proteins influencing how proteins move through secretory pathways.
Biological function summary
GOLPH2 plays an essential role in maintaining Golgi function and efficient protein trafficking. It does not belong to a large protein complex but its proper functioning is critical for handling secretory pathways. The protein keeps secretory flow organized which affects cellular responses and interactions with the environment. The GOLPH2 protein also has connections to immunity responding to stimuli like infections or inflammation.
Pathways
GOLPH2 actively participates in secretory pathways and protein transport systems. Its role includes coordination with other Golgi proteins to facilitate protein modification and sorting. GOLPH2 interacts with other proteins like GOLM1 and GOLPH3 impacting the Golgi's structural organization and influencing pathways like vesicular transport. These interactions are important for adapting the intracellular environment to external demands.
GOLPH2 levels change in diseases such as liver cancer and hepatitis. These changes can serve as biomarkers for diagnosis and disease progression monitoring. GOLPH2 also connects with proteins involved in these diseases including AFP a known tumor marker. Understanding GOLPH2's role in such conditions aids research in developing diagnostic tools and therapeutic strategies.