Abcam, ab277439, Human CD35 ELISA Kit

Size: 1 x 96Tests
Human CD35 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human CD35 in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.2 ng/mL,
Range:0.205 - 50 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human CD35 ELISA Kit (ab277439) is an
in-vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human CD35 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human CD35 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human CD35 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human CD35 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human CD35 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C