Abcam, ab277440, Human PAR1/Thrombin Receptor ELISA Kit

Size: 1 x 96Tests
Human PAR1/Thrombin Receptor ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human PAR1/Thrombin Receptor in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 3 pg/mL,
Range:2.87 - 700 pg/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human PAR1/Thrombin Receptor ELISA Kit (ab277440) is an
in-vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human PAR1/Thrombin Receptor in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human PAR1/Thrombin Receptor coated on a 96-well plate. Standards and samples are pipetted into the wells and Human PAR1/Thrombin Receptor present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human PAR1/Thrombin Receptor antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human PAR1/Thrombin Receptor bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C