Abcam, ab277453, Human Junctional Adhesion Molecule 2/JAM-B ELISA Kit

Size: 1 x 96Tests
Human Junctional Adhesion Molecule 2/JAM-B ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Junctional Adhesion Molecule 2/JAM-B in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.068 ng/mL,
Range:0.069 - 50 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Junctional Adhesion Molecule 2/JAM-B ELISA Kit (ab277453) is an
in-vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human Junctional Adhesion Molecule 2/JAM-B in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Junctional Adhesion Molecule 2/JAM-B coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Junctional Adhesion Molecule 2/JAM-B present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Junctional Adhesion Molecule 2/JAM-B antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Junctional Adhesion Molecule 2/JAM-B bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Junctional Adhesion Molecule 2 also known as JAM-B JAM B or JAM Protein is a member of the immunoglobulin superfamily of proteins. It has a molecular mass of approximately 38 kDa and is expressed on the surface of endothelial and epithelial cells. JAM-B plays an important role in regulating cellular adhesion facilitating the interaction between adjacent cells. This target is important in maintaining cell polarity and integrity making it an adhesion example of the body’s cellular architecture.
Biological function summary
Junctional adhesion molecule 2 participates in a range of processes related to the formation and maintenance of tight junctions. It is a component of the tight junction complex interacting with other proteins to influence barrier function in vascular and epithelial tissues. This molecule is involved in leukocyte transmigration which is essential during inflammation as leukocytes need to exit the bloodstream to reach sites of infection or injury. It partners with other tight junction proteins which assists in its role in cell adhesion dynamics.
Pathways
JAM-B is involved in the regulation of the leukocyte adhesion cascade and tight junction signaling. Its function is interlinked with the integrin and chemokine pathways influencing leukocyte migration. Junctional adhesion molecule 2 associates with proteins like integrins and PECAM-1 playing a part in the orchestration of cellular movements and interactions that are critical to proper immune response and tissue homeostasis.
Junctional adhesion molecule 2 correlation exists with inflammatory diseases and cancer. Its role in leukocyte transmigration links it to inflammatory conditions where increased or disrupted JAM-B expression can affect immune cell infiltration and inflammation severity. In cancer aberrant JAM-B expression can influence tumor growth and metastasis due to its impact on cell adhesion and migration. JAM-B interacts with PD-1 in the context of these diseases highlighting its part in immune regulation and signaling networks associated with tumor progression.